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DNA Damage by Free Radical Production by Aminoguanidine
Author(s) -
SUJI GEORGE,
SIVAKAMI SUBRAMANIUM
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1354.023
Subject(s) - glycation , hydrogen peroxide , scavenger , chemistry , dna damage , hydroxyl radical , free radical scavenger , incubation , dna , free radical theory of aging , pharmacology , biochemistry , radical , medicine , oxidative stress , receptor
Aminoguanidine (AG), a prototype therapeutic dicarbonyl scavenger, is the most potent drug available today to inhibit the formation of advanced glycation endproducts (AGEs) and to reverse glycation‐mediated damage in normal aging. This paper examines the ability of AG to cause damage to supercoiled plasmid DNA in the presence of the transition metal, Fe +3 . Damage to DNA was dependent on the concentrations of both the transition metal and AG. We could detect hydroxyl radical as well as hydrogen peroxide during the incubation of AG with Fe +3 . Thus this finding further cautions against the indiscriminate use of AG in clinical prophylaxis in diabetes and questions its use as a therapeutic agent.