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The Effects of Urotensin‐II on Proliferation of Pheochromocytoma Cells and mRNA Expression of Urotensin‐II and Its Receptor in Pheochromocytoma Tissues
Author(s) -
ZENG ZHENGPEI,
LIU GUOQIANG,
LI HANZHONG,
FAN XINRONG,
LIU DONGMEI,
TONG ANLI,
ZHENG XIN,
LIU CHANG
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1353.032
Subject(s) - urotensin ii , pheochromocytoma , receptor , medicine , endocrinology , messenger rna , biology , cell growth , gene , genetics
Abstract: This study was conducted to investigate the effects of urotensin‐II (UII) on the proliferation of pheochromocytoma cells and the mRNA expression of UII and its receptor G protein–coupled receptor 14 (GPR14) in normal adrenal and human pheochromocytoma tissues. The effects on the cell proliferation by different UII concentrations and at different time were observed with the MTT method in both rat pheochromocytoma cell line (PC12 cell) and human pheochromocytoma cells. The mRNA expression of UII and GPR14 was evaluated by RT‐PCR. UII had no significant effect on the proliferation in PC12 cells, but promoted the proliferation of human pheochromocytoma cells. The mRNA expression of UII and GPR14 in pheochromocytoma was lower than that in normal adrenal tissues. But mRNA expression of UII and GPR14 in adrenal pheochromocytomas was lower than that in extra‐adrenal pheochromocytomas. Thus, UII and its receptor GPR14 exert a possible effect in the pathogenesis of pheochromocytoma.