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Mechanism of PNA Transport to the Nuclear Compartment
Author(s) -
STANKOVA LENKA,
ZIEMBA AMY J.,
ZHILINA ZHANNA V.,
EBBINGHAUS SCOT W.
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1348.064
Subject(s) - digitonin , nucleic acid , biochemistry , nucleus , cell nucleus , chemistry , biophysics , peptide , microbiology and biotechnology , biology , gene , membrane
We evaluated the nuclear uptake of fluorescently labeled peptide nucleic acids and measured the binding of unlabeled peptide nucleic acids (PNAs) to the endogenous HER‐2/neu promotor in digitonin‐permeabilized SK‐BR‐3 cells. Fluorescently labeled PNAs readily enter the nucleus of digitonin‐permeabilized cells, and binding to the chromosomal target sequence was detected with a bis‐PNA. Nuclear uptake and target sequence binding were inhibited by N‐ethylmaleimide (NEM) and GTPγS. We conclude that PNAs are transported into the nucleus through an energy‐dependent process involving the nuclear pore complex.