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Mechanical Loading Differentially Regulates Membrane‐Bound and Soluble RANKL Availability in MC3T3‐E1 Cells
Author(s) -
KIM DAE WON,
LEE HAHNJUN,
KARMIN JAIME A.,
LEE SE EUN,
CHANG SEONGSIL,
TOLCHIN BEN,
LIN SHIPHIN,
CHO SAM K.,
KWON ANTHONY,
AHN JAE MOK,
LEE FRANCIS YOUNGIN
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1346.054
Subject(s) - rankl , chemistry , transfection , microbiology and biotechnology , biophysics , biochemistry , receptor , biology , gene , activator (genetics)
To understand the biochemical response of RANKL in response to mechanical loading, MC3T3‐E1 cells were biequiaxially stretched. A murine RANKL cDNA with double epitopes, pEF6 HA‐RANKL‐V5His, was transfected into MC3T3‐E1 cells, which were then stretched. Endogenous RANKL protein expression increased in response to mechanical loading. Membrane‐bound RANKL (HA‐RANKL‐V5His) increased in cell lysates while soluble RANKL (RANKL‐V5His) decreased in the conditioned media after mechanical loading. This may have resulted from the decreased activity of TACE after mechanical loading. Increased membrane‐bound RANKL may be one of the mechanisms through which osteoblasts adapt to mechanical loading by regulating osteoclastogenic activity in a region‐specific manner.