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Fluorescent Properties of Spinach Leaf Plasma Membranes and Chloroplast Envelopes
Author(s) -
CVETIĆ TIJANA,
VUČINIĆ ŽELJKO
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1342.078
Subject(s) - membrane , chloroplast , fluorescence , spinach , chloroplast membrane , excited state , chemistry , analytical chemistry (journal) , plasma , excitation , photochemistry , flavin group , aqueous solution , biophysics , thylakoid , chromatography , biology , biochemistry , optics , physics , atomic physics , quantum mechanics , gene , enzyme
A bstract : Plasma membranes and chloroplast envelopes were isolated from green spinach leaves, and emission and excitation spectra recorded in aqueous solution at 25°C. Chloroplast envelopes excited by 420 nm showed strong emission peaks at 635 and 680 nm that came from chlorophyll precursors present only in these membranes. Upon UV excitation, both plasma membranes and chloroplast envelopes exhibited emission peak at 420 nm originating from pterins and 520 nm due to flavins. Oxidation of the membranes increased both the emission and excitation fluorescence intensity.