Premium
Ascorbic Acid and the Oxidative Processes in Pea Root Cell Wall Isolates: Characterization by Fluorescence and EPR Spectroscopy
Author(s) -
VELJOVIĆJOVANOVIĆ SONJA,
KUKAVICA BILJANA,
CVETIĆ TIJANA,
MOJOVIĆ MILOŠ,
VUČINIĆ ŽELJKO
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1342.076
Subject(s) - chemistry , ascorbic acid , electron paramagnetic resonance , radical , fluorescence , hydroxyl radical , photochemistry , oxygen , cell wall , fluorescence spectroscopy , biochemistry , organic chemistry , nuclear magnetic resonance , physics , food science , quantum mechanics
A bstract : A comparative fluorescence and oxygen radical‐sensitive spin trap EPR spectroscopic study of isolated cell walls (with proteins or deproteinated), in the presence and absence of ascorbate and H 2 O 2 is presented. Fluorescence spectra indicate the presence of at least two fluorophores, one degraded and the other synthesized after reduction or oxidation, indicating phenol/di/polymerization. DEPMPO spin trap measurements show that isolated cell walls are capable of oxygen‐dependent hydroxyl radical generation in the absence of NADH or other reductants, ascorbate addition, or deproteination of the cell wall abolishing the signal due to hydroxyl radicals.