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Characterization of Enzymatically Synthesized Diferulate
Author(s) -
TUFEGDŽIĆ SRÐAN,
BOGDANOVIĆ JELENA,
MAKSIMOVIĆ VUK,
VUČINIĆ ŽELJKO
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1342.068
Subject(s) - horseradish peroxidase , fluorescence , substrate (aquarium) , chromophore , chemistry , excited state , molecule , emission spectrum , characterization (materials science) , photochemistry , spectral line , analytical chemistry (journal) , materials science , chromatography , nanotechnology , enzyme , organic chemistry , optics , physics , oceanography , astronomy , nuclear physics , geology
A bstract : Horseradish peroxidase was used to synthesize diferulates by a procedure in which ethyl ferulate was used as substrate. Four different forms were obtained, of which two dominant were the 5‐5′ and 8‐5′ diferulate. Fluorescence emission spectra of the diferulates (excited at 284 nm) indicate that they contain two chromophores, as opposed to the substrate molecule. Fluorescence excitation spectra with emission at 417 nm further demonstrate the difference between the synthesized diferulates and starting substrates.