Premium
Imaging of Spiking and Subthreshold Activity of Mitral Cells with Voltage‐Sensitive Dyes
Author(s) -
DJURIŠIĆ MAJA,
ZEČEVIĆ DEJAN
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1342.009
Subject(s) - soma , dendrite (mathematics) , tuft , olfactory bulb , dendritic spike , neuroscience , axon , biophysics , chemistry , glomerulus , electrophysiology , anatomy , biology , central nervous system , materials science , excitatory postsynaptic potential , inhibitory postsynaptic potential , geometry , mathematics , kidney , endocrinology , composite material
A bstract : To obtain a more complete description of individual neurons, it is necessary to complement electrical measurements with technologies such as voltage imaging with intracellular dyes, which permit massive parallel recording from many sites on neuronal processes. Utilizing such an approach, we investigate the functional structure of the mitral cell, the principal output neuron in the rat olfactory bulb. These experiments were designed to determine the number, location, and the stability of spike trigger zones, the excitability of terminal dendritic branches, the pattern and nature of spike initiation and propagation in the primary dendrite, the basic characteristics of the evoked EPSPs at the site of origin (the glomerular tuft), and its attenuation along the primary dendrite. The images of spike trigger zones showed that an action potential can be initiated in three different compartments of the mitral cell: the soma‐axon region, the primary dendrite trunk, and the terminal dendritic tuft, which appears to be fully excitable. The amplitude of the EPSPs evoked by olfactory nerve stimulation was determined at the site of origin (glomerular tuft) and its attenuation was monitored optically along the entire length of the primary dendrite.