Premium
Prevention of Cellular Oxidative Damage by an Aqueous Extract of Anoectochilus formosanus
Author(s) -
WANG LENGFANG,
LIN CHUNMAO,
SHIH CHWENMING,
CHEN HUIJU,
SU BORCHERNG,
TSENG CHENGCHUANG,
GAU BAOBIH,
CHENG KURTA
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1338.060
Subject(s) - oxidative stress , chemistry , hydrogen peroxide , xanthine oxidase , antioxidant , apoptosis , reactive oxygen species , dna fragmentation , xanthine , dna damage , biochemistry , fragmentation (computing) , oxidative phosphorylation , poly adp ribose polymerase , programmed cell death , polymerase , dna , biology , enzyme , ecology
A bstract :Anoectochilus formosanus (AF) is a popular folk medicine in Taiwan whose pharmacological effects have been characterized. In this work we investigated the antioxidant properties of an aqueous extract prepared from AF. The AF extract was capable of scavenging H 2 O 2 in a dose‐dependent manner. We induced oxidative stress in HL‐60 cells, either by the addition of hydrogen peroxide (H 2 O 2 ) or by the xanthine/xanthine oxidase reaction. Apoptosis caused by oxidative damage was displayed by DNA fragmentation on gel electrophoresis, and the apoptotic fraction was quantified with flow cytometry. The cell damage induced by oxidative stress was prevented by the plant extract in a concentration‐dependent manner. Furthermore, the proteolytic cleavage of poly(ADP‐ribose) polymerase during the apoptotic process was also inhibited by AF extract. Our results provide the basis for determining an AF extract to be an antioxidant.