Enhancement of Cisplatin‐Induced Apoptosis and Caspase 3 Activation by Depletion of Mitochondrial DNA in a Human Osteosarcoma Cell Line | Zendy

YEN HSIUCHUAN | Zendy; TANG YICHIA | Zendy; CHEN FANYI | Zendy; CHEN SHIHWEI | Zendy; MAJIMA HIDEYUKI J. | Zendy

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Enhancement of Cisplatin‐Induced Apoptosis and Caspase 3 Activation by Depletion of Mitochondrial DNA in a Human Osteosarcoma Cell Line

Author(s) -

YEN HSIUCHUAN,

TANG YICHIA,

CHEN FANYI,

CHEN SHIHWEI,

MAJIMA HIDEYUKI J.

Publication year - 2005

Publication title -

annals of the new york academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.712

H-Index - 248

eISSN - 1749-6632

pISSN - 0077-8923

DOI - 10.1196/annals.1338.047

Subject(s) - cisplatin , apoptosis , dna fragmentation , cytochrome c , mitochondrial dna , programmed cell death , fragmentation (computing) , mitochondrion , microbiology and biotechnology , cell culture , cancer research , chemistry , cytosol , caspase , caspase 3 , biology , biochemistry , genetics , enzyme , chemotherapy , gene , ecology

A bstract : Cisplatin is an anticancer drug that can induce apoptosis. In this study, we investigated the effect of mitochondrial DNA (mtDNA) depletion on cisplatin‐induced cell death using a human osteosarcoma cell line (143B) and mtDNA‐depleted 143B cells (143B‐ρ 0 ). Results showed that cisplatin decreased cell survival in 143B‐ρ 0 cells. Moreover, cisplatin induced a greater extent of apoptosis‐associated DNA fragmentation and caspase 3 activation in 143B‐ρ 0 cells. The release of mitochondrial cytochrome c into cytosol by cisplatin was enhanced more obviously in 143B cells than in 143B‐ρ 0 cells; however, in the control group of 143B‐ρ 0 cells, it was already dramatically greater. Depletion of mtDNA may increase sensitivity of cells to cisplatin‐induced apoptosis by enhancing caspase 3 activation via both cytochrome c ‐dependent and ‐independent pathways.

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