Premium
Angiogenic Potency of Amadori‐Glycated Phosphatidylethanolamine
Author(s) -
NAKAGAWA KIYOTAKA,
OAK JEONGHO,
MIYAZAWA TERUO
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1333.048
Subject(s) - glycation , amadori rearrangement , umbilical vein , angiogenesis , phosphatidylethanolamine , chemistry , matrix metalloproteinase , potency , endocrinology , medicine , diabetes mellitus , biochemistry , pharmacology , in vitro , phospholipid , phosphatidylcholine , membrane
A bstract : Glycation has been thought to participate in diabetic vascular diseases. However, there are no reports about the effects of lipid glycation on endothelial dysfunction. In this study, we have evaluated whether Amadori‐glycated phosphatidylethanolamine (Amadori‐PE), a lipid‐linked glycation compound, affected proliferation, migration, and tube formation of cultured human umbilical vein endothelial cells. These three factors involved in angiogenesis were significantly stimulated by Amadori‐PE at a low concentration of less than 5 μM. Furthermore, Amadori‐PE also stimulated the secretion of matrix metalloproteinase‐2 (MMP‐2), a pivotal enzyme in the initial step of angiogenesis. Our results indicated for the first time that Amadori‐PE would elicit vascular disease through angiogenic potency on endothelial cells, thereby playing an active part in the development and progression of diabetic microangiopathy.