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Characterization of Cellular Uptake and Distribution of Vitamin E
Author(s) -
SAITO YOSHIRO,
YOSHIDA YASUKAZU,
NISHIO KEIKO,
HAYAKAWA MIEKO,
NIKI ETSUO
Publication year - 2004
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1331.047
Subject(s) - chemistry , tocotrienol , vitamin e , tocopherol , selenium , biochemistry , fractionation , oxidative stress , antioxidant , chromatography , organic chemistry
A bstract : We previously reported that tocotrienols acted as more potent inhibitors against selenium deficiency‐induced cell death than the corresponding tocopherol isoforms ( J. Biol. Chem. 2003;278:39428‐39434). In the present study, we first compared the differences in the cellular uptake between α‐tocopherol (α‐Toc) and α‐tocotrienol (α‐Toc‐3). The initial rate of cellular uptake of α‐Toc‐3 was 70‐fold higher than that of α‐Toc. Subcellular fractionation analysis of α‐Toc‐3 and α‐Toc‐fortified cells showed similar cellular distribution of these antioxidants, which was directly proportional to the lipid distribution. The cells containing similar amounts of α‐Toc‐3 and α‐Toc showed similar resistance against the oxidative stress caused by peroxides. These results suggest that the apparent higher cytoprotective effect of α‐Toc‐3 than α‐Toc is primarily ascribed to its higher cellular uptake.