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Vitamin E and Gene Expression in Immune Cells
Author(s) -
HAN SUNG NIM,
ADOLFSSON OSKAR,
LEE CHEOLKOO,
PROLLA TOMAS A.,
ORDOVAS JOSE,
MEYDANI SIMIN NIKBIN
Publication year - 2004
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1331.010
Subject(s) - immune system , t cell , cd28 , biology , cd8 , gene expression , vitamin d and neurology , gene , apoptosis , microarray analysis techniques , calcitriol receptor , cell cycle , signal transduction , microbiology and biotechnology , major histocompatibility complex , immunology , endocrinology , genetics
A bstract : Aging is associated with dysregulation of immune cells, particularly T cells. Previous studies indicated that vitamin E improves T cell function, in part by a direct effect on T cells. We studied gene expression profile of T cells to better understand the underlying mechanisms of aging‐ and vitamin E‐induced changes in T cell function. Young and old C57BL mice were fed diets containing 30 (control) or 500 (E) ppm of vitamin E for 4 weeks. T cells were purified from splenocytes by negative selection using magnetic beads (anti‐Mac‐1 and anti‐MHC class II), then cultured with media or stimulated with anti‐CD3 and anti‐CD28. Gene expression profile was assessed using microarray analysis. Genes showing more than two‐fold changes, P < 0.05 by ANOVA, and with at least one present call were selected. Aging had significant effects on genes involved in signal transduction, transcriptional regulation, and apoptosis pathways in T cells, while vitamin E had a significant effect on genes associated with the regulation of cell cycle.