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Tetramethoxy Hydroxyflavone p7F Downregulates Inflammatory Mediators via the Inhibition of Nuclear Factor κB
Author(s) -
LEE HEEGU,
KIM HYOSON,
OH WONKEUN,
YU KYUNGAE,
CHOE YONGKYUNG,
AHN JONGSEOG,
KIM DONGSOO,
KIM SOOHYUN,
DINARELLO CHARLES A.,
KIM KILHYOUN,
YOON DOYOUNG
Publication year - 2004
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1329.065
Subject(s) - proinflammatory cytokine , chemistry , nitric oxide , prostaglandin e2 , tumor necrosis factor alpha , pharmacology , nitric oxide synthase , lipopolysaccharide , nf κb , reactive oxygen species , in vivo , inflammation , biochemistry , immunology , medicine , biology , signal transduction , endocrinology , organic chemistry , microbiology and biotechnology
A bstract :Artemisia has been traditionally used in Korean herbal medicine to clear damp heat and to treat uteritis and jaundice. Flavonoids isolated from Artemisia are also known to possess anti‐inflammatory activities. In this study, 5,6,3′,5′‐tetramethoxy 7,4′‐hydroxyflavone (p7F) was isolated from Artemisia absinthium . We examined in vitro and in vivo regulatory functions of p7F on the production of nitric oxide (NO), prostaglandin E 2 (PGE 2 ), and tumor necrosis factor‐α (TNF‐α) as well as the expression of inducible NO synthase (iNOS), cyclooxygenase‐2 (COX‐2), and collagen‐induced arthritis. p7F inhibited the expression or production of proinflammatory mediators such as COX‐2/PGE 2 and iNOS/NO in lipopolysaccharide (LPS)‐stimulated RAW 264.7 cells. p7F also suppressed the serum level of TNF‐α in mice treated with collagen and inhibited nuclear factor‐κB (NF‐κB) activation as well as NF‐κB promoter activity in RAW 264.7 cells stimulated with LPS. This compound directly inhibited the intracellular accumulation of reactive oxygen species in hydrogen peroxide‐stimulated RAW 264.7 cells. p7F has antioxidant activity and inhibits NF‐κB activation. Taken together, these results suggest that p7F can be clinically applied to the treatment of inflammatory diseases.