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Brain Expression of Tyrosine Hydroxylase and Its Regulation by Steroid Hormones in the European Eel Quantified by Real‐Time PCR
Author(s) -
WELTZIEN FA,
PASQUALINI C,
LE BELLE N,
VIDAL B,
VERNIER P,
DUFOUR S.
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1327.108
Subject(s) - tyrosine hydroxylase , forebrain , endocrinology , olfactory bulb , medicine , hindbrain , dopamine , biology , cerebrum , hormone , midbrain , in situ hybridization , testosterone (patch) , gene expression , central nervous system , biochemistry , gene
A bstract : In the eel, dopamine inhibits pubertal development. To investigate the regulatory mechanisms involved, we developed a quantitative real‐time RT‐PCR assay for measurement of brain expression of tyrosine hydroxylase (TH), the rate‐limiting enzyme in the biosynthesis of dopamine. TH expression was highest in the olfactory bulb, followed by the di‐/mesencephalic areas and the telencephalon/preoptic area. TH expression in the optic lobes and hindbrain was low or below the detection limit. In vivo treatment with testosterone, but not estradiol, resulted in increased TH expression in the forebrain, except the optic tectum, but not in the hindbrain. The results were confirmed by in situ hybridization.