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Regulation of Thyroid Hormone Availability by Iodothyronine Deiodinases at the Blood‐Brain Barrier in Birds
Author(s) -
VERHOELST C H J,
GEYTEN S,
ROELENS S A,
DARRAS V M
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1327.103
Subject(s) - deiodinase , iodothyronine deiodinase , polyclonal antibodies , endocrinology , medicine , hormone , thyroid , dio2 , downregulation and upregulation , immunocytochemistry , choroid plexus , antiserum , biology , chemistry , antibody , thyroid hormones , biochemistry , central nervous system , gene , immunology
A bstract : It is accepted that type II iodothyronine deiodinase (D2) is predominantly found in brain, where it maintains homeostasis of thyroid hormone (TH) levels. The current study describes the production of a polyclonal D2 antiserum and its use in the comparison of D2 protein distribution with that of type I (D1) and type III (D3) deiodinase protein in the chicken choroid plexus (CP). Immunocytochemistry showed high D2 protein expression in the epithelial cells of the CP, whereas the D1 and D3 proteins were absent. Furthermore, dexamethasone treatment led to an upregulation of the D2 protein in these cells.