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The Use of Real‐Time PCR to Study the Expression of Thyroid Hormone Receptor β2 in the Developing Chicken
Author(s) -
GROMMEN SYLVIA V. H.,
GROEF BERT,
KÜHN EDUARD R.,
DARRAS VEERLE M.
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1327.053
Subject(s) - taqman , thyroid hormone receptor , receptor , messenger rna , in situ hybridization , thyroid , hormone , real time polymerase chain reaction , biology , microbiology and biotechnology , hormone receptor , thyroid hormone receptor beta , endocrinology , biochemistry , gene , genetics , cancer , breast cancer
A bstract : Thyroid hormones and their receptors (TRs) have critical functions in development and metabolism. In chicken, three TRs are known: TRα, TRβ0, and TRβ2. The latter was isolated from chicken eye, but its presence in other tissues has not yet been extensively investigated. We therefore developed a real‐time PCR assay using a Taqman probe and primers based on the unique amino‐terminal region of TRβ2. We detected a strong TRβ2 mRNA signal in the pituitary, confirmed with in situ hybridization, and in several other tissues. TRβ2 mRNA was more abundant in the pituitary of newly hatched chicks than in 15‐day‐old embryos.