The Advantage of Absolute Quantification in Comparative Hormone Research as Indicated by a Newly Established Real‐Time RT‐PCR: GH, IGF‐I, and IGF‐II Gene Expression in the Tilapia,  Oreochromis niloticus | Zendy

EPPLER ELISABETH | Zendy; CAELERS ANTJE | Zendy; BERISHVILI GIORGI | Zendy; REINECKE M.NFRED | Zendy

Premium

The Advantage of Absolute Quantification in Comparative Hormone Research as Indicated by a Newly Established Real‐Time RT‐PCR: GH, IGF‐I, and IGF‐II Gene Expression in the Tilapia, Oreochromis niloticus

Author(s) -

EPPLER ELISABETH,

CAELERS ANTJE,

BERISHVILI GIORGI,

REINECKE M.NFRED

Publication year - 2005

Publication title -

annals of the new york academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.712

H-Index - 248

eISSN - 1749-6632

pISSN - 0077-8923

DOI - 10.1196/annals.1327.047

Subject(s) - real time polymerase chain reaction , gene , gene expression , growth hormone , primer (cosmetics) , biology , tilapia , cloning (programming) , messenger rna , microbiology and biotechnology , computational biology , hormone , fish <actinopterygii> , genetics , chemistry , computer science , endocrinology , organic chemistry , fishery , programming language

A bstract : We have developed a real‐time RT‐PCR that absolutely quantifies the gene expression of hormones using the standard curve method. The method avoids cloning procedures by using primer extension to create templates containing a T7 promoter gene sequence. It is rapid since neither separate reverse transcriptions nor postamplification steps are necessary, and its low detection level (2 pg/μg total RNA) allows precise absolute quantification. Using the method, we have quantified the gene expression of GH, IGF‐I, and IGF‐II in the tilapia.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research