Premium
Upregulation of the Somatotropic Axis Is Correlated with Increased G6PDH Expression in Black Sea Bream Adapted to Iso‐osmotic Salinity
Author(s) -
DEANE EDDIE E.,
WOO NORMAN Y. S.
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1327.045
Subject(s) - salinity , osmoregulation , biology , growth hormone , gene expression , insulin like growth factor , seawater , endocrinology , somatotropic cell , osmotic concentration , chemistry , medicine , zoology , hormone , growth factor , gene , biochemistry , ecology , receptor
A bstract : Black sea bream ( Mylio macrocephalus ) were adapted to salinities of 0 ppt (freshwater), 6 ppt (hypo‐osmotic), 12 ppt (iso‐osmotic), 33 ppt (seawater), and 50 ppt (hypersaline) for 1 month. Using RT‐PCR assays, the expression of pituitary growth hormone ( GH ) and hepatic insulin‐like growth factor 1 ( IGF‐1 ) genes were studied. It was found that the transcripts for both of these genes were highest in fish maintained at iso‐osmotic salinity. To correlate the expression of GH and IGF‐1 with an index of growth, we also measured the transcript levels of glucose‐6‐phosphate dehydrogenase ( G6PDH ) in liver. It was found that this transcript was also elevated in iso‐osmotically adapted black sea bream.