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Growth Hormone Increases hsc70/hsp70 Expression and Protects against Apoptosis in Whole Blood Preparations from Silver Sea Bream
Author(s) -
DEANE EDDIE E.,
WOO NORMAN Y. S.
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1327.044
Subject(s) - hsp70 , apoptosis , growth hormone , dna fragmentation , heat shock protein , gene expression , biology , gene , hormone , camptothecin , andrology , microbiology and biotechnology , endocrinology , medicine , chemistry , programmed cell death , biochemistry
A bstract : Preparations of whole blood, from silver sea bream ( Sparus sarba ), were used for heat shock protein 70 (hsp70) and apoptosis studies. It was found that the expression of both gene members of the hsp70 family ( hsc70 and hsp70 ) were upregulated during acute heat shock. The transcript abundance of both of these genes was increased when cells were exposed to growth hormone (GH) at concentrations of 10 and 100 ng/mL. It was also found that GH at concentrations of 10 and 100 ng/mL could protect whole blood from camptothecin‐induced apoptosis as determined by DNA fragmentation analysis.