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Cloning and Expression of Guanylin‐like Peptides in Teleost Fish
Author(s) -
CRAMB GORDON,
MARTINEZ ANNESOPHIE,
McWILLIAM IAIN S.,
WILSON GILLIAN D.
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1327.042
Subject(s) - cloning (programming) , fish <actinopterygii> , microbiology and biotechnology , expression (computer science) , chemistry , fishery , biology , computer science , programming language
A bstract : Homologues of the guanylin peptide family are expressed in teleost fish. Using the eel as a model euryhaline species, cDNAs for three peptides, guanylin, uroguanylin, and renoguanylin, were cloned and found to be expressed within both renal and intestinal epithelia. Seawater (SW) acclimation resulted in upregulation of uroguanylin mRNA expression in the intestinal epithelia of both immature “yellow” and sexually mature “silver” eels. SW acclimation also resulted in an increase in guanylin mRNA in silver eel intestine, whereas there was no change in renoguanylin mRNA expression under any condition. No changes in expression were found in the kidney following SW acclimation.