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Analysis of Xenopus Melanotrope Cell Size and POMC‐Gene Expression
Author(s) -
CORSTENS G J H,
ROUBOS E W,
JENKS B G,
ERP P E J
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1327.040
Subject(s) - xenopus , flow cytometry , gene expression , gene , microbiology and biotechnology , cell , biology , messenger rna , real time polymerase chain reaction , andrology , chemistry , medicine , genetics
A bstract : Flow cytometry was used to separate Xenopus melanotrope cells according to their size. The cells were then submitted to real‐time RT‐PCR to determine the level of POMC‐gene expression. The results show a positive correlation between cell size and gene expression for cells from black‐background (but not white‐background) adapted animals.