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In Situ Hybridization Localization of TRH Precursor and TRH Receptor mRNAs in the Brain and Pituitary of Xenopus laevis
Author(s) -
GALAS L,
BIDAUD I,
BULANT M,
JENKS B G,
OUWENS D T W M,
JÉGOU S,
LADRAM A,
ROUBOS E W,
NICOLAS P,
TO M C,
VAUDRY H
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1327.012
Subject(s) - xenopus , in situ hybridization , chemistry , in situ , receptor , microbiology and biotechnology , medicine , endocrinology , neuroscience , messenger rna , biology , biochemistry , gene , organic chemistry
A bstract : We examined the distribution of the mRNAs encoding proTRH and the three TRH receptor subtypes ( x TRHR1, x TRHR2, and x TRHR3) in the Xenopus laevis CNS and pituitary. A positive correlation was generally observed between the expression patterns of proTRH and x TRHR mRNAs. x TRHRs were widely expressed in the telencephalon and diencephalon, where two or even three x TRHR mRNAs were often simultaneously observed within the same brain structures. In the pituitary, x TRHR2 was selectively expressed in the distal lobe, and x TRHR3 was found exclusively in the intermediate lobe of white background‐adapted animals, indicating that, in amphibians, the effect of TRH on α‐melanotropin (α‐MSH) secretion from melanotrope cells is mediated through the novel receptor subtype x TRHR3.