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Nucleation of Insulin Crystals in a Wide Continuous Supersaturation Gradient
Author(s) -
PENKOVA ANITA,
DIMITROV IVAYLO,
NANEV CHRISTO
Publication year - 2004
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1324.006
Subject(s) - supersaturation , nucleation , insulin , materials science , chemical physics , chemistry , medicine , organic chemistry
A bstract : Modifying the classical double pulse technique, by using a supersaturation gradient along an insulin solution contained in a glass capillary tube, we found conditions appropriate for the direct measurement of nucleation parameters. The nucleation time lag has been measured. Data for the number of crystal nuclei versus the nucleation time were obtained for this hormone. Insulin was chosen as a model protein because of the availability of solubility data in the literature. A comparison with the results for hen‐egg‐white lysozyme, HEWL was performed.