Premium
Extracellular Nucleic Acids in Cultures of Long‐Term Cultivated Eukaryotic Cells
Author(s) -
MOROZKIN EVGENIY S.,
LAKTIONOV PAVEL P.,
RYKOVA ELENA Y.,
VLASSOV VALENTIN V.
Publication year - 2004
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1318.038
Subject(s) - nucleic acid , hela , rna , dna , cell , cell culture , chemistry , microbiology and biotechnology , biochemistry , biology , genetics , gene
A bstract : Investigation of the kinetics of nucleic acid release by HeLa (human cervical carcinoma cell line) and A431 (human squamous carcinoma cell line) cells is presented. The released DNA and RNA were shown to accumulate in culture medium and at the cell surface. A portion of cell surface bound RNA can be eluted with PBS/EDTA. Mild trypsin treatment is required for complete detachment of cell surface bound RNA and cell surface bound DNA. Electrophoretic analysis reveals characteristic patterns of cell‐associated and free RNA and DNA molecules.