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Extracellular DNA in Breast Cancer: Cell‐Surface‐Bound, Tumor‐Derived Extracellular DNA in Blood of Patients with Breast Cancer and Nonmalignant Tumors
Author(s) -
RYKOVA ELENA YU.,
LAKTIONOV PAVEL P.,
SKVORTSOVA TATYANA E.,
STARIKOV ANDREY V.,
KUZNETSOVA NINA P.,
VLASSOV VALENTIN V.
Publication year - 2004
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1318.033
Subject(s) - breast cancer , extracellular , dna , dna methylation , cancer research , fibroadenoma , cancer , polymerase chain reaction , methylation , microbiology and biotechnology , chemistry , breast fibroadenoma , gene , pathology , biology , medicine , biochemistry , gene expression
A bstract : A methylation‐specific polymerase chain reaction technique was used to investigate aberrant promoter methylation of RASSF1A and HIC‐1 genes in circulating extracellular DNA (exDNA) from the blood of breast cancer and fibroadenoma patients. Methylated DNA could be detected in the exDNA eluted from the surface of erythrocytes and leukocytes, even in the samples where no methylated DNA could be detected in plasma. The data obtained demonstrate that cell surface bound exDNA provides a valuable source of material for early noninvasive cancer diagnostics and monitoring.