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Detection of Cell‐Free DNA in Bronchial Lavage Fluid Supernatants of Patients with Lung Cancer
Author(s) -
CARSTENSEN TIM,
SCHMIDT BERND,
ENGEL EILEEN,
JANDRIG BURKHARD,
WITT CHRISTIAN,
FLEISCHHACKER MICHAEL
Publication year - 2004
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1318.031
Subject(s) - dna , nucleic acid , polymerase chain reaction , cell , microbiology and biotechnology , cell free fetal dna , lung cancer , pathology , chemistry , biology , medicine , biochemistry , gene , genetics , pregnancy , fetus , prenatal diagnosis
A bstract : Recently, it was shown that it is possible to isolate free circulating DNA from plasma/serum of patients with benign and malignant diseases. In addition, several groups were able to detect tumor‐associated alterations in these nucleic acids. We wondered whether any nucleic acids are detectable in cell‐free bronchial lavage supernatants, which until now have been discarded after cell harvest. Additionally, we wanted to find out if it is possible to detect tumor‐associated alterations in these DNA molecules. DNA was isolated from cell‐free lavage supernatants from 30 lung cancer patients, and the DNA was examined for microsatellite alterations. Intact DNA could be isolated from all cell‐free bronchial lavage supernatants. Microsatellite alterations were found in lavage supernatants of 12 of 30 patients and in lavage cells of 6 of 30 patients. Altogether, alterations were found in 14 of 30 patients. Thus, we could demonstrate for the first time that it is possible to isolate intact DNA from cell‐free bronchial lavage supernatants. Their quantity and quality are sufficient for further amplification via polymerase chain reaction. Altogether, tumor‐associated changes were detected in the DNA of 47% of the patients that were analyzed.