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Transcession of DNA from Bacteria to Human Cells in Culture: A Possible Role in Oncogenesis
Author(s) -
ANKER PHILIPPE,
ZAJAC VLADIMIR,
LYAUTEY JACQUELINE,
LEDERREY CHRISTINE,
DUNAND CHRISTOPHE,
LEFORT FRANÇOIS,
MULCAHY HUGH,
HEINEMANN JACK,
STROUN MAURICE
Publication year - 2004
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1318.030
Subject(s) - bacteria , bacillus subtilis , organism , microorganism , dna , biology , microbiology and biotechnology , cell culture , polymerase chain reaction , carcinogenesis , gene , hl60 , genetics
A bstract : The human organism is continuously in close contact with microorganisms, especially bacteria. In the present work, by means of a real‐time polymerase chain reaction (PCR) technique, we looked for the presence of a distinct bacterial gene in human cells. To this end, we cultured a human cell line, HL60, in a supernatant in which bacteria ( Bacillus subtilis ) had been grown. A transient transcession of bacterial DNA into the human cells was observed.