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Detection of Promoter Hypermethylation of Tumor Suppressor Genes in Urine from Kidney Cancer Patients
Author(s) -
CAIRNS PAUL
Publication year - 2004
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1318.007
Subject(s) - suppressor , dna methylation , cancer , gene , tumor suppressor gene , cancer research , urine , kidney cancer , kidney , biology , medicine , oncology , genetics , carcinogenesis , gene expression
A bstract : Kidney cancer is curable by surgical resection and therapy, if detected at an early stage. Using sensitive methylation‐specific polymerase chain reaction, we screened matched tumor DNA and preoperative urine DNA from 50 kidney cancer patients, for hypermethylation of a panel of six normally unmethylated tumor suppressor genes: VHL, p16/CDKN2a, p14ARF, APC, RASSF1A, and Timp‐3 . When compared to the tumor DNA, an identical pattern of gene hypermethylation was found in the matched urine DNA from 44 of 50 patients (88% sensitivity) including 27 of 30 cases of stage I disease. By contrast, hypermethylation was not observed in normal and benign disease controls (100% specificity). We conclude that promoter hypermethylation is a common and early event in kidney tumorigenesis and can be detected in the urine DNA from patients with organ‐confined renal cancer of all histologic types.