Premium
PACAP Stimulates Biosynthesis and Release of Endozepines from Rat Astrocytes
Author(s) -
MASMOUDIKOUKI OLFA,
GANDOLFO PIERRICK,
LEPRINCE JEROME,
VAUDRY DAVID,
PELLETIER GEORGES,
FOURNIER ALAIN,
VAUDRY HUBERT,
TO MARIECHRISTINE
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1317.094
Subject(s) - adenylyl cyclase , vasoactive intestinal peptide , adenylate kinase , neuropeptide , downregulation and upregulation , chemistry , receptor , cyclase , astrocyte , intracellular , protein kinase c , endocrinology , medicine , camp dependent pathway , microbiology and biotechnology , signal transduction , biology , biochemistry , gene , central nervous system
Astrocytes synthesize and release endozepines, a family of neuropeptides related to diazepam‐binding inhibitor (DBI). Astroglial cells also express the receptors of pituitary adenylate cyclase‐activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP). In the present article, we show that PACAP dose dependently increases DBI gene expression and stimulates endozepine release through activation of PAC1‐R. PACAP increases cAMP formation, enhances polyphosphoinositide turnover, and evokes calcium mobilization from intracellular Ca 2+ pools. The effect of PACAP on endozepine release is mediated through the adenylyl cyclase/PKA pathway while the downregulation of astrocyte response to PACAP can be ascribed to activation of the PLC/PKC pathway.