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PACAP Stimulates the Release of Interleukin‐6 in Cultured Rat Müller Cells
Author(s) -
SEKI T.,
HINOHARA Y.,
TAKI C.,
NAKATANI M.,
OZAWA M.,
NISHIMURA S.,
TAKAKI A.,
ITHO H.,
TAKENOYA F.,
SHIODA S.
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1317.043
Subject(s) - in vivo , cell culture , bromodeoxyuridine , microbiology and biotechnology , in vitro , receptor antagonist , medicine , receptor , cell growth , biology , endocrinology , chemistry , antagonist , antiserum , interleukin , retina , cytokine , immunology , neuroscience , biochemistry , antibody , genetics
We have investigated the in vivo effect of PACAP on rat Müller cells that are the predominant glial element in the retina. Müller cells were treated with PACAP38, either alone or in the presence of the PACAP‐selective antagonist, PACAP6–38. Cellular proliferation was determined by measuring the incorporation of bromodeoxyuridine, while interleukin‐6 (IL‐6) levels in the culture medium were examined using a B9 cell bioassay. In cultured rat Müller cells, the expression of PACAP receptor (PAC1‐R) was assessed with immunohistochemistry using a PAC1‐R‐specific antiserum. PACAP stimulated IL‐6 production in Müller cells at a concentration as low as 10 −12 M, which was not sufficient to induce cell proliferation. This elevation of IL‐6 production was significantly inhibited by PACAP6–38. These data suggest that Müller cells are one of the target cells for PACAP, stimulating the release of IL‐6, and providing a mechanism whereby PACAP exerts a significant neuroprotective effect in the retina.