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PACAP Stimulates the Release of the Secretogranin II‐Derived Peptide EM66 from Chromaffin Cells
Author(s) -
GUILLEMOT JOHANN,
AITALI DJIDA,
TURQUIER VALERIE,
MONTEROHADJADJE MAITE,
FOURNIER ALAIN,
VAUDRY HUBERT,
ANOUAR YOUSSEF,
YON LAURENT
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1317.033
Subject(s) - chromaffin cell , stimulation , protein kinase c , adrenal medulla , chemistry , endocrinology , medicine , microbiology and biotechnology , peptide , signal transduction , biology , biochemistry , catecholamine
The aim of the present article was to examine the effect of PACAP on the release of the SgII‐derived peptide EM66 from primary cultures of bovine chromaffin cells. PACAP dose dependently stimulated EM66 release from cultured chromaffin cells. A significant response was observed after 6 h of treatment with PACAP and increased to reach a 3.6‐fold stimulation at 72 h. The stimulatory effect of PACAP was mediated through multiple signaling pathways, including calcium influx through L‐type channels, PKA, PKC, and MAP‐kinase cascades, to regulate EM66 release from chromaffin cells. These data suggest that EM66 may act downstream of the trans‐synaptic stimulation of the adrenal medulla by neurocrine factors.