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Expression and GTP Sensitivity of Peptide Histidine Isoleucine High‐Affinity‐Binding Sites in Rat
Author(s) -
DEBAIGT COLIN,
MEUNIER ANNIECLAIRE,
GOURSAUD STEPHANIE,
MONTONI ALICIA,
PINEAU NICOLAS,
COUVINEAU ALAIN,
LABURTHE MARC,
MULLER JEANMARC,
JANET THIERRY
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1317.017
Subject(s) - gtp' , isoleucine , histidine , guanosine , peptide , vasoactive intestinal peptide , binding site , chemistry , biochemistry , receptor , methionine , amino acid , microbiology and biotechnology , biology , leucine , neuropeptide , enzyme
High‐affinity‐binding sites for the vasoactive intestinal peptide (VIP) analogs peptide histidine/isoleucine‐amide (PHI)/carboxyterminal methionine instead of isoleucine (PHM) are expressed in numerous tissues in the body but the nature of their receptors remains to be elucidated.The data presented indicate that PHI discriminated a high‐affinity guanosine 5′‐triphosphate (GTP)‐insensitive‐binding subtype that represented the totality of the PHI‐binding sites in newborn rat tissues but was differentially expressed in adult animals. The GTP‐insensitive PHI/PHM‐binding sites were also observed in CHO cells over expressing the VPAC2 but not the VPAC1 VIP receptor.