Premium
PACAP and VIP Promote Initiation of Electrophysiological Activity in Differentiating Embryonic Stem Cells
Author(s) -
CHAFAI MAGDA,
LOUISET ESTELLE,
BASILLE MAGALI,
CAZILLIS MICHÈLE,
VAUDRY DAVID,
ROSTÈNE WILLIAM,
GRESSENS PIERRE,
VAUDRY HUBERT,
GONZALEZ BRUNO J.
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1317.010
Subject(s) - vasoactive intestinal peptide , electrophysiology , neuropeptide , microbiology and biotechnology , embryonic stem cell , adenylate kinase , biology , receptor , neuroscience , pituitary adenylate cyclase activating peptide , in vitro , chemistry , biochemistry , gene
Owing to their capacity to differentiate in vitro into various types of neuronal cells, embryonic stem (ES) cells represent a suitable model for studying the first steps of neuronal differentiation and cerebral development. Since pituitary adenylate cyclase‐activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP) are known to control maturation of the nervous system, we have investigated the possible effects of these two neuropeptides on the differentiation of ES cells. Reverse transcription polymerase chain reaction (RT‐PCR) analysis revealed that mouse ES cells express PAC1 and VPAC2 receptors.Electrophysiological recordings demonstrated that PACAP and VIP facilitate the emission of currents, suggesting that these peptides can initiate the genesis of an electrophysiological activity in differentiating ES cells.