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Effects of VIP and VIP–DAP on Proliferation and Lipid Peroxidation Metabolism in Human KB Cells
Author(s) -
CARAGLIA MICHELE,
DICITORE ALESSANDRA,
GIUBERTI GAIA,
CASSESE DIANA,
LEPRETTI MARILENA,
CARTENÌ MARIA,
ABBRUZZESE ALBERTO,
STIUSO PAOLA
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1317.007
Subject(s) - lipid peroxidation , lipid metabolism , metabolism , chemistry , oxidative metabolism , biochemistry , medicine , oxidative stress
In the present study, we have utilized the transglutaminase (TGase) enzyme to modify the primary structure of VIP with diamminopropane (DAP) at the level of the Gln16. We have investigated the conformational stability of VIP and VIP–DAP in solution by limited proteolysis experiments. The VIP–DAP appears to be more resistant to the proteolytic attack of trypsin, thus indicating that the derivatization in position 16 is able to stabilize the structure of the peptide. However, we have studied their role in cell cycle modulation and antioxidant activity in the oropharyngeal epidermoid carcinoma KB cells.