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The Anti‐Alpha‐Actinin Test Completes Anti‐DNA Determination in Systemic Lupus Erythematosus
Author(s) -
CROQUEFER S,
RENAUDINEAU Y,
JOUSSE S,
GUEGUEN P,
ANSART S,
SARAUX A,
YOUINOU P
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1313.017
Subject(s) - lupus nephritis , medicine , iif , antibody , nephritis , immunology , rheumatoid arthritis , alpha (finance) , anti dsdna antibodies , pathogenesis , lupus erythematosus , autoantibody , disease , construct validity , nursing , patient satisfaction
A bstract : In murine systemic lupus erythematosus (SLE) models, nephritogenic anti‐dsDNA IgG has been shown to cross‐react with a kidney antigen, alpha‐actinin, and to be critical in renal pathogenesis. In humans, studies of anti‐alpha‐actinin antibodies (Abs) are scarce, and these antibodies remain to be evaluated. We have thus far tested sera from patients with SLE ( n = 103 ), rheumatoid arthritis (RA, n = 93 ), and primary Sjögren syndrome (pSS, n = 34 ), and from healthy subjects ( n = 160 ), for the presence of anti‐alpha‐actinin and anti‐DNA Abs. The latter were tested using several methods [IIF on Crithidia luciliae (Crit) and ELISA using dsDNA]. Anti‐alpha‐actinin Abs were confirmed by Western blot. Sera from 23 of 103 SLE patients, 3 of 93 RA patients, 1 of 33 pSS patients, and 1 of 160 controls scored positive for anti‐alpha‐actinin Abs. In SLE, the positivity was significantly associated with anti‐dsDNA reactivity (22 of 23): 19 of 23 sera were alpha‐actinin‐positive/dsDNA‐positive and 13 were alpha‐actinin‐positive/Crit‐positive. Few cases were alpha‐actinin/positive/dsDNA‐negative: 1 SLE, 3 RA, and 1 control. Furthermore, anti‐alpha‐actinin Abs have been detected at high level before or at the early stage of lupus nephritis when compared with active and inactive SLE without kidney manifestations.