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Microarrays and the Gene Expression Profile of a Single Cell
Author(s) -
KAWASAKI ERNEST S.
Publication year - 2004
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1310.010
Subject(s) - dna microarray , gene expression , computational biology , gene , biology , microarray , gene expression profiling , cell , single cell analysis , genetics
A bstract : With completion of the human genome sequence, it is now possible to study the expression of the entire human gene vcomplement of ∼30,000‐35,000 genes. To accomplish this goal, microarrays have become the leading methodology for the analysis of global gene expression. Improvements in technology have increased the sensitivity of microarrays to the point where it is feasible to study gene expression in a small number of cells and even at the single cell level . A summary of developments in the area of expression profiling in single cells will be described, and the rationale for these types of studies will be presented. In addition, from a biologist's point of view, some bioinformatic challenges of expression analysis of single cells will be discussed.