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Agmatine Inhibits Naloxone‐Induced Contractions in Morphine‐Dependent Guinea Pig Ileum
Author(s) -
ARICIOGLU FEYZA,
ERCIL ESER,
DULGER GUL
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1304.016
Subject(s) - agmatine , yohimbine , morphine , (+) naloxone , idazoxan , ileum , chemistry , pharmacology , guinea pig , endocrinology , medicine , opioid , antagonist , biochemistry , arginine , prazosin , receptor , amino acid
A bstract : This study investigates the effects of agmatine on naloxone‐precipitated withdrawal syndrome in morphine‐dependent guinea pig ileum. Male guinea pigs that were starved for 24 hours were decapitated after cervical dislocation, and terminal portions of the ilea were removed. Segments were fixed at a resting tension of 1 g in an organ bath containing 1 3 10 26 M morphine in Tyrode solution at 378C, which was bubbled with 95% O 2 and 5% CO 2 . Tissues were incubated in morphine containing Tyrode solution for 4 hours before agmatine was added. Naloxone and agmatine had no effect on naive ilea. Naloxone (1 3 10 26 M) contracted morphine‐dependent ilea. Agmatine significantly inhibited the contractile response to naloxone in a dose‐dependent manner (1 3 10 27 M, 44%; 1 3 10 26 M, 80%; 1 3 10 25 M, 95%). This effect of agmatine was partly abolished by pretreatment with yohimbine and was almost completely abolished by idazoxan.