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Glycoxidation of Low‐Density Lipoprotein Increases TUNEL Positivity and CPP32 Activation in Human Coronary Cells
Author(s) -
NIGRIS FILOMENA,
TAJANA GIANFRANCO,
CONDORELLI MARIO,
D'ARMIENTO FRANCESCO P.,
SICA GIACOMO,
LERMAN LILACH O.,
NAPOLI CLAUDIO
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1299.128
Subject(s) - tunel assay , apoptosis , immunocytochemistry , medicine , diabetes mellitus , endocrinology , chemistry , lipoprotein , glycosylation , western blot , low density lipoprotein , biochemistry , cholesterol , gene
A bstract : Apoptosis of arterial cells induced by oxidized low‐density lipoprotein (oxLDL) is thought to contribute to the progression of vascular dysfunction and atherogenesis. It is well established that diabetes mellitus is accompanied by both glycosylation and oxidation of LDL (glc‐oxLDL), but the biological effects of these modified lipoproteins are poorly understood. We demonstrate here for the first time that glc‐oxLDL increases TUNEL positivity and caspase‐3 activation (by Western blot and immunocytochemistry) of human coronary smooth muscle cells. Overall, these effects induced by glc‐oxLDL were greater than those achieved with oxLDL. Thus, glc‐oxLDL activated downstream apoptotic signaling. This may influence the evolution of atherogenesis and vascular complications in diabetes.