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Surfactant Protein D Binds Genomic DNA and Apoptotic Cells, and Enhances Their Clearance, in Vivo
Author(s) -
PALANIYAR NADES,
CLARK HOWARD,
NADESALINGAM JEYA,
HAWGOOD SAMUEL,
REID KENNETH B.M.
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1299.085
Subject(s) - collectin , surfactant protein d , dna , apoptosis , microbiology and biotechnology , in vivo , chemistry , cell , biology , innate immune system , biochemistry , receptor , genetics
A bstract : Collectins, such as surfactant protein D (SP‐D), bind apoptotic cells; however, the ligands that they recognize on these cells are unknown. We hypothesized that SP‐D binds to the DNA present on these cells. We show that SP‐D binds and aggregates mouse alveolar macrophage DNA effectively. Alveolar macrophages of SP‐D (−/−) mice contained more nicked DNA than those of SP‐A (−/−) and wild type mice. Our results also suggest that carbohydrate recognition domains of SP‐D may recognize DNA present on the apoptotic cells. Therefore, cell‐surface DNA could be a ligand for recognition of apoptotic cells by collectins.