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Rescue of Cells from Apoptosis by Antioxidants Occurs Downstream from GSH Extrusion
Author(s) -
LIUZZI FRANCESCA,
FANELLI CLAUDIA,
CIRIOLO MARIA ROSA,
CERELLA CLAUDIA,
D'ALESSIO MARIA,
DENICOLA MILENA,
MAGRINI ANDREA,
BERGAMASCHI ANTONIO,
GHIBELLI LINA
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1299.080
Subject(s) - apoptosis , chemistry , microbiology and biotechnology , downstream (manufacturing) , oxidative stress , radical , antioxidant , glutathione , extrusion , reactive oxygen species , biophysics , biochemistry , biology , materials science , enzyme , operations management , metallurgy , economics
A bstract : Antioxidants‐that is, scavengers of free radicals and anaerobic conditions (5%CO 2 , 95% N 2 )‐protect monocytic U937 cells from damage‐induced apoptosis. Antioxidants rescue the cells acting on the apoptotic pathway at a step downstream from gluthatione extrusion. Reducing agents, such as DTT, also reduce stress‐induced apoptosis. Thus, apoptotic GSH extrusion triggers the downstream events of apoptosis by leaving cells unprotected against thiols oxidation and radical production.