Premium
Accumulation of Histones in Cell Lysates Precedes Expression of Apoptosis‐Related Phagocytosis Signals in Human Lymphoblasts
Author(s) -
GABLER CHRISTOPH,
BLANK NORBERT,
WINKLER SILKE,
KALDEN JOACHIM R.,
LORENZ HANNSM
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1299.039
Subject(s) - lymphoblast , apoptosis , phagocytosis , phosphatidylserine , histone , programmed cell death , dna fragmentation , biology , microbiology and biotechnology , lupus erythematosus , autoimmunity , cell , immune system , immunology , chemistry , cell culture , dna , antibody , biochemistry , membrane , genetics , phospholipid
A bstract : Systemic lupus erythematosus (SLE) is characterized by the production of autoantibodies directed against several nuclear components, such as DNA and histones. Apoptosis was induced in activated human lymphoblasts ( n = 6 ) by UV‐B irradiation for 30 sec followed by continuous culturing. An extranuclear accumulation of the nucleosomal histones H2A, H2B, H3, and H4 in cell lysates was observed very early in the process of apoptosis, even before phosphatidylserine externalization occurred on the outer membrane surface of apoptotically dying lymphoblasts. We hypothesize that a dysregulation of apoptosis during these early phases may contribute to the induction of autoimmunity against nuclear autoantigens as seen in SLE.