Premium
Prodigiosin Induces Apoptosis by Acting on Mitochondria in Human Lung Cancer Cells
Author(s) -
LLAGOSTERA ESTHER,
SOTOCERRATO VANESSA,
MONTANER BEATRIZ,
PÉREZTOMÁS RICARDO
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1299.030
Subject(s) - apoptosis , prodigiosin , cytochrome c , mitochondrion , cell culture , cytotoxicity , serratia marcescens , cytosol , microbiology and biotechnology , apoptosis inducing factor , programmed cell death , biology , caspase , cancer cell , chemistry , biochemistry , cancer research , cancer , in vitro , enzyme , genetics , escherichia coli , gene
A bstract : Prodigiosin (PG) is a secondary metabolite, isolated from a culture of Serratia marcescens , which has shown potent cytotoxicity against various human cancer cell lines as well as immunosuppressive activity. The purpose of this study was to evaluate the role of mitochondria in PG‐induced apoptosis. Therefore, we evaluated the apoptotic action of PG in GLC4 small cell lung cancer cell line by Hoechst 33342 staining. In these cells, we examined mitochondrial apoptosis‐inducing factor (AIF) and cytochrome c (cyt c ) release to the cytosol in PG time‐response studies. These findings suggest that PG induces apoptosis in both caspase‐dependent and caspase‐independent pathways.