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Repeated Immobilization Stress Decreases mRNA and Protein Levels of the Type 1 IP 3 Receptor in Rat Heart
Author(s) -
KREPSOVA KATARINA,
MICUTKOVA LUCIA,
NOVOTOVA MARTA,
KUBOVCAKOVA LUCIA,
KVETNANSKY RICHARD,
KRIZANOVA OLGA
Publication year - 2004
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1296.042
Subject(s) - receptor , western blot , endocrinology , medicine , messenger rna , gene expression , reverse transcription polymerase chain reaction , chemistry , real time polymerase chain reaction , biology , microbiology and biotechnology , gene , biochemistry
A bstract : Stress is one of the major contributors to the development of cardiovascular disorders and psychiatric illnesses. Immobilization stress belongs to severe stressors and is known to activate several calcium transport systems. The aim of this work was to determine whether repeated immobilization stress changes mRNA and protein levels of the type 1 and 2 inositol‐1,4,5‐trisphosphate (IP 3 ) receptors in cardiac tissue. Rats were immobilized for 7 days, 2 h daily. After repeated immobilization, increased numbers of collagen fibers were accumulated in the heart atria compared to hearts of the control group of rats. Gene expression was determined after reverse transcription and subsequent real‐time polymerase chain reaction, using SYBR Green fluorescent dye. Protein levels were determined by Western blot and hybridization with the primary antibody against IP 3 receptors. Contrary to single immobilization, repeated immobilization decreased a gene expression of the type 1 and 2 IP 3 receptors, and also protein levels of the IP 3 receptors. Although the physiologic relevance of our observations remains to be elucidated, we propose that the decrease in IP 3 receptors may have an impact on the development of the pathophysiologic changes in the heart.