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Mutation Scan of a Type 1 Diabetes Candidate Gene
Author(s) -
NOLSØE RÚNA L.,
POCIOT FLEMMING,
NOVICK DANIELA,
RUBINSTEIN MENACHEM,
KIM SOOHYUN,
DINARELLO CHARLES A.,
MANDRUPPOULSEN THOMAS
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1288.053
Subject(s) - biology , exon , genetics , untranslated region , allele , intron , immune system , gene , pathogenesis , proinflammatory cytokine , candidate gene , microbiology and biotechnology , immunology , messenger rna , inflammation
A bstract : Type 1 (insulin‐dependent) diabetes, T1DM, is the result of an immune‐mediated destruction of the pancreatic β cells dependent mainly on T helper cells and macrophages. Interleukin‐18 (IL‐18) is a proinflammatory cytokine produced mainly by macrophages. IL‐18 is capable of inducing T lymphocyte synthesis of IFNγ, thereby skewing the T helper response toward a T helper type 1 (Th1) profile. IL‐18 binding protein (IL18BP) neutralizes IL‐18 and leads to a reduced Th1 response. Polymorphisms in IL18BP may affect the activity of IL‐18 and the magnitude of the Th1 response and may play a role in the pathogenesis of T1DM. The aim of the study was therefore to identify polymorphisms in IL18BP and to test these for association with T1DM. We evaluated the human IL18BP gene on chromosome 11q13 as a candidate susceptibility gene for T1DM and scanned the entire IL18BP (promoter, exons 1‐6, and 3′UTR) for polymorphisms using single‐strand conformational polymorphism analysis and direct sequencing. We identified a total of 11 polymorphisms, all having allele frequencies ranging between 0.05 and 0.10. Four were in the 5′UTR: −257G→T, −78C→T, −65G→A, and −59A→G. Three were in intron 3: IVS3+140A→C, IVS4−147G→T, and IVS4−59G→T. The last four, 38*A→T, 48*T→A, 388*C→G, and 440*_441*insG, were in the 3′UTR of IL18BP. However, none of these were frequent enough to permit association studies in T1DM and we conclude that IL18BP does not contribute to the overall genetic susceptibility to type 1 diabetes.