Premium
Development and Validation of a Radioligand Binding Assay to Measure Insulin Specific IgG Subclass Antibodies in Human Serum
Author(s) -
FINCOKENT D.,
MORRONE A.,
MOXNESS M.,
BEDIAN V.,
KRASNER A.,
FOLEY J.,
STENE M.,
KAWABATA T.
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1288.039
Subject(s) - subclass , radioligand , radioligand assay , antibody , chemistry , immunoassay , immunoglobulin g , microbiology and biotechnology , immunology , biology , biochemistry , receptor
A bstract : The objective was to develop and validate a radioligand binding assay for insulin antibodies (IABs) of the IgG1, IgG2, IgG3, and IgG4 subclasses in human serum. The validation studies focused on determining specificity, capacity, linearity, sensitivity, and precision of each assay. It was seen that our assay for IAB IgG subclasses is specific and has sufficient capacity to measure each of the subclasses in human serum. Moreover, the linear region and limits of detection and quantitation for each assay are clearly determined.