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Relaxin Downregulates the Calcium Binding Protein S100A4 in MDA‐MB‐231 Human Breast Cancer Cells
Author(s) -
RADESTOCK YVONNE,
HOANGVU CUONG,
HOMBACHKLONISCH SABINE
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1282.070
Subject(s) - relaxin , estrogen receptor , downregulation and upregulation , cancer research , breast cancer , estrogen , estrogen receptor beta , extracellular , transfection , endocrinology , chemistry , receptor , cell culture , cancer cell , estrogen receptor alpha , medicine , cancer , microbiology and biotechnology , biology , biochemistry , gene , genetics
A bstract : Expressed in the human breast and in human breast cancer tissues, the heterodimeric peptide hormone relaxin is involved in extracellular matrix turnover. To investigate the role of relaxin in estrogen receptor‐alpha negative human breast cancer cells, we established transfectants of the human MDA‐MB‐231 breast cancer cell line stably overexpressing H2‐relaxin (MDA‐MB‐231/pIRES‐EGFP‐H2). These transfectants produced and secreted functional relaxin. Our investigations revealed downregulation of mRNA and protein of the calcium binding protein S100A4 (metastasin) in MDA‐MB‐231/pIRES‐EGFP‐H2 transfectants, indicating a distinct and novel role for relaxin in estrogen receptor‐alpha negative human breast cancer cells.