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Relaxin Stimulates cAMP Production in MCF‐7 Cells upon Overexpression of Type V Adenylyl Cyclase
Author(s) -
NGUYEN BAO T.,
DESSAUER CARMEN W.
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1282.044
Subject(s) - relaxin , adenylyl cyclase , adcy10 , chemistry , gs alpha subunit , mcf 7 , adcy9 , medicine , endocrinology , microbiology and biotechnology , receptor , biology , biochemistry , human breast , cancer , cancer cell
A bstract : Relaxin stimulates cAMP production and activation of ERK and PI3K in THP‐1 cells. Relaxin also stimulates protein kinase C zeta (PKCζ) translocation to the plasma membrane in a PI3K‐dependent manner in THP‐1 and MCF‐7 cells. However, relaxin did not increase cAMP production in MCF‐7 cells. We overexpressed different adenylyl cyclase (AC) isoforms in MCF‐7 cells to examine coupling of endogenous relaxin receptors to cAMP production. Overexpression of types II and IV AC had no effect on cAMP production by relaxin. However, overexpression of type V AC, which is activated by PKCζ, showed synergistic stimulation of cAMP by relaxin and forskolin.