Premium
Signal Switching after Stimulation of LGR7 Receptors by Human Relaxin 2
Author(s) -
HALLS MICHELLE L.,
BATHGATE ROSS A.,
SUMMERS ROGER J.
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1282.042
Subject(s) - adenylate kinase , relaxin , stimulation , signal transduction , phosphorylation , receptor , microbiology and biotechnology , cyclase , protein kinase a , kinase , biology , intracellular , chemistry , biochemistry , endocrinology
A bstract : Previous studies have described a biphasic cAMP response after stimulation of LGR7 by human gene 2 (H2) relaxin, involving both adenylate cyclase and PI3‐kinase activity. The current study identifies the upstream involvement of G i in the PI3‐kinase‐mediated response, likely the result of receptor signal switching. Amino acid sequence analysis of the LGR7 C‐terminal tail and intracellular loops revealed multiple putative phosphorylation sites, suggesting that signal switching from G s to G i may occur after receptor phosphorylation. This study supports a time‐dependent biphasic cAMP response: an initial short G s ‐adenylate cyclase‐mediated cAMP response is followed by receptor signal switching to a G i ‐PI3‐kinase‐mediated response.