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Central Effects of Long‐Term Relaxin Expression in the Rat
Author(s) -
SILVERTOWN JOSH D.,
FRASER RUSSELL,
POTERSKI ROMAN S.,
GEDDES BRAD,
SUMMERLEE ALASTAIR J.S.
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1282.033
Subject(s) - relaxin , vasopressin , endocrinology , medicine , gene expression , immunofluorescence , recombinant dna , andrology , biology , transduction (biophysics) , chemistry , antibody , gene , immunology , biochemistry , hormone
A bstract : A recombinant adenovirus containing the human H2 preprorelaxin (hH2) cDNA and a reporter gene was coinjected with a transactivator virus (Ad‐tTA) into the lateral cerebral ventricles of female rats. Cardiovascular effects were measured over a 21‐day period. Circulating vasopressin in the periphery was significantly greater ( P <.0001) in the relaxin‐treated group throughout the experimental period, compared with controls. There was a significant decrease in plasma osmolality ( P <.05) by approximately 10 mmol/L in the treated group by day 14. Immunofluorescence for hH2 present in cryosections showed rAd transduction and hH2 expression from ependymal cells of the ventricular system. Adenovirus‐mediated delivery of hH2 to the brain is capable of producing bioactive relaxin that affects cardiovascular parameters.